FIG. 5.
Binding of gD1(234t) to immobilized HveAt. (A) Sensorgram overlays. (B) Maximum koff determination using the dissociation phase of the sensorgram at 16 μM gD1(234t). Squares show the data for the first 10 s of dissociation, and the straight line is the fit to the initial second of data. The slope of the line gives a maximum koff of 2 × 10−1 s−1. r2 for the linear fit is 0.989. (C) Scatchard analysis. Equilibrium binding levels (RU bound) were obtained from sensorgrams in panel A. C is the molar concentration of gD1(234t). The negative inverse of the slope of the line gives a KD of 2 × 10−5 M. r2 for the linear fit is 0.978.