TABLE 3.
Kinetic and affinity values for gDt-HveAt complex formation
| Protein | kon (103 s−1 M−1) | koff (10−2 s−1) | KD (10−6 M) (koff/kon)e |
|---|---|---|---|
| gD1(306t)a | 6.1 ± 0.6b | 2.0 ± 0.2b | 3.2 ± 0.6 |
| gD1(Δ290-299t)a | 240 ± 70b | 0.78 ± 0.1b | 0.033 ± 0.01 |
| gD1(Δ277-299t) | 160 | 0.97 | 0.061 |
| gD1(Δ277-290t) | 31 | 1.5 | 0.48 |
| gD1(285t) | 300 | 1.1 | 0.037 |
| gD1(275t) | 180 | 0.74 | 0.041 |
| gD1(234t) | NDc | 20d | 20f |
a
Values for gD1(306t) and gD1(Δ290-299t) were reported previously (44) and are shown here for comparison.
b
Average ± standard deviation from at least three separate experiments.
c
ND, not determined.
d
Estimated maximum koff (see Fig. 5B).
e
χ2 values for the global fits ranged from 1.1 to 4.5.
f
From Scatchard analysis (see Fig. 5C).