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. 2024 Mar 20;96(13):5289–5297. doi: 10.1021/acs.analchem.4c00215

Table 3. Accuracy of the Premix Strategy Using Whatman 1 Paper for SB-PS-MS Analysis with LC-MS Quantification as the Real Value: Human Plasmaa.

AA [AA]human plasma analysisLC-MS(μM) [AA]human premix Whatman1 PS-MS(μM) bias premixWhatman 1–LC (%)
Gly 156 140 10
Ala 153 183 19
Ser 90.1 92.5 11
Pro 89.5 102 13
Val 93.3 102 10
Thr 93.1 97.9 5
Leu + Ile 57.2 61.5 8
Asp   21.2  
Phe 35.3 34 12
Arg 152 131 14
Tyr 43.7 47.4 9
a

The [AA]human premix Whatman 1 PS-MS (μM) represents the average result from three replicates. For each replicate, the bias was calculated, and the results are presented as bias premixWhatman 1–LC (%), which is the average of the bias calculations from the three replicates.