Table 3. Accuracy of the Premix Strategy Using Whatman 1 Paper for SB-PS-MS Analysis with LC-MS Quantification as the Real Value: Human Plasmaa.
| AA | [AA]human plasma analysisLC-MS(μM) | [AA]human premix Whatman1 PS-MS(μM) | bias premixWhatman 1–LC (%) |
|---|---|---|---|
| Gly | 156 | 140 | 10 |
| Ala | 153 | 183 | 19 |
| Ser | 90.1 | 92.5 | 11 |
| Pro | 89.5 | 102 | 13 |
| Val | 93.3 | 102 | 10 |
| Thr | 93.1 | 97.9 | 5 |
| Leu + Ile | 57.2 | 61.5 | 8 |
| Asp | 21.2 | ||
| Phe | 35.3 | 34 | 12 |
| Arg | 152 | 131 | 14 |
| Tyr | 43.7 | 47.4 | 9 |
The [AA]human premix Whatman 1 PS-MS (μM) represents the average result from three replicates. For each replicate, the bias was calculated, and the results are presented as bias premixWhatman 1–LC (%), which is the average of the bias calculations from the three replicates.