FIG. 4.

ATP-Mg-dependent tegument release in vitro is inhibited by the presence of phosphatase. Purified wt virions were incubated with ATP-Mg in the presence or absence of CIP for 30 min; addition of phosphatase inhibited VP13/14 and VP22 release. Note the absence of higher-MW forms of VP22 in the phosphatase-treated sample. A third sample was incubated without phosphatase for 30 min and then incubated in the presence of phosphatase for 30 min; the levels of VP13/14 and VP22 in the released (R) fraction were unaffected, although the presence of higher-MW VP22 forms was abolished. P, pelleted material.