FIG. 7.

CKII but not PKA can promote VP22 release in UL13 mutant virions in vitro. ATP-Mg-dependent VP22 release in vitro was severely impaired in purified UL13 mutant virions. The effect of adding exogenous CKII (A) or PKA (B) in conjunction with ATP-Mg to incubations of UL13 mutant virions was therefore studied. The samples were fractionated as before and analyzed by SDS-PAGE followed by Western blotting with R205. The amount of soluble VP22 was greater in the presence than on the absence of CKII. VP13/14 release appeared unaffected. Addition of PKA had no visible effect on VP22 or VP13/14 release compared to a mixture lacking this enzyme. P and R, pelleted and released material, respectively.