Table 2. Design of the Experiments and the Obtained Results.

trial no.	X1 rpm	X2 min	X3%	X4 ng/mL	EE (mean ± SD) %	trial no.	X1 rpm	X2 min	X3%	X4 ng/mL	EE (mean ± SD) %
1	550	90	1.0	200	56.2 ± 0.25	15	900	90	0.7	300	72.6 ± 0.04
2	200	90	0.7	200	62.9 ± 0.18	16	550	90	0.7	250	69.5 ± 0.05
3	200	30	0.7	250	68.0 ± 0.50	17	550	30	1.0	250	66.6 ± 0.14
4	200	150	0.7	250	81.0 ± 0.35	18	200	90	1.0	250	77.6 ± 0.25
5	550	90	0.4	300	78.2 ± 0.42	19	900	30	0.7	250	65.4 ± 0.13
6	200	90	0.7	300	84.5 ± 0.44	20	550	150	1.0	250	66.9 ± 0.48
7	900	90	0.7	200	50.6 ± 0.10	21	550	150	0.7	200	54.7 ± 0.10
8	900	150	0.7	250	58.4 ± 0.25	22	900	90	0.4	250	61.7 ± 0.14
9	550	30	0.7	200	47.0 ± 0.18	23	550	150	0.4	250	66.5 ± 0.12
10	550	90	0.4	200	53.0 ± 0.19	24	550	90	1.0	300	76.8 ± 0.37
11	900	90	1.0	250	66.1 ± 0.005	25	200	90	0.4	250	74.5 ± 0.18
12	550	90	0.7	250	71.0 ± 0.17	26	550	150	0.7	300	76.5 ± 0.30
13	550	30	0.7	300	74.2 ± 0.18	27	550	30	0.4	250	62.1 ± 0.23
14	550	90	0.7	250	70.9 ± 0.19