FIGURE 3.

Tyr 105 site of PKM2 interacts with SHP2 in GC cells. (A) Immunoprecipitation (IP) was revealed by SDS‐PAGE‐silver staining or Coomassie brilliant blue staining. (B) The IP‐coupled shotgun LC–MS of the protein product screened the proteins that may bind to SHP2. (C) We adopted HADDOCK 2.4 to perform the protein–protein docking. ⁴⁸ Protein docking showed the possibility and potential binding sites of SHP2 and PKM2 binding. (D). Sequence comparison reveals a quite pronounced across the sequence conservation in PKM2‐Y105. (E–G) Cell lysate from HGC‐27 cells were incubated with SHP2 antibody (Ab) or PKM2 Ab or IgG, and then subjected to WB analysis with SHP2 Ab, PKM2 Ab, or p‐PKM2‐Y105 Ab.