FIGURE 6.

SHP099 suppressed the proliferation and migration of GC cells, and SHP2/PKM2/AMPK forms a positive feedback loop in GC. (A) The effect of different doses of SHP099 on the phosphorylation level of SHP2, PKM2, AMPK, and ERK in MKN‐45 cells. (B and C) Effects of SHP099 at different concentrations on the clonal forming ability and migratory ability of HGC‐27 cells. (D) The influence of SHP099 on different metabolic indexes in MKN‐45 cells. (E) Compound C reversed the effect of SHP099 on AMPK phosphorylation level in MKN‐45 cells. (F) Compound C reversed the effect of SHP099 on migration ability of HGC‐27 cells. (G) Compound C activated SHP2 was verified by WB in HGC‐27 cells. **p < 0.01.