FIGURE 7.

DDP activated SHP2 in GC cells. (A–C) The influence of different stimuli on the activation state of SHP2 in HGC‐27 cells. (D) WB analysis of the phosphorylation expression of SHP2, AMPK, H2A in HGC‐27 cells. (E) γH2A.X staining in the indicated groups was imaged via immunofluorescence in HGC‐27 cells. (F) The DDP+SHP099 group had the higher sensitivity displaying lower half maximal inhibitory concentration (IC50) than DDP alone group in GC cells by CCK8 assay. (G) Evaluation of the rate of proliferating cells after treated with DDP or DDP+SHP099 by EdU assay. Scale bar = 200 µm.