Table 3.

The primers employed for identifying Cryptosporidium spp., and subtypes of C. parvum, C. viatorum, C. felis in the present study

Usage (s)	Primer sequence (5′ to 3′)	Fragment length (bp)	Annealing temperature (°C)	Gene	Ref
Cryptosporidium genus specific	18SiCF2: GACATATCATTCAAGTTTCTGAC 18SiCR2: CTGAAGGAG TAAGGAACAACC 18SiCF1: CCTATCAGCTTTAGACGGTAGG 18SiCR1: TCTAAGAATTTCACCTCTGACTG	∼ 587	58 58	SSU rRNA	[17]
Subtyping of C. parvum	AGP-F1: ATAGTCTCCGCTGTATTC AGP-R1: GGAAGGAACGATGTATCT AGP-F2: TCCGCTGTATTCTCAGCC AGP-R2: GCAGAGGAACCAGCATC	∼ 850	55 58	gp60	[9]
Subtyping of C. viatorum	CviatF2: TTCATTCTGACCCCTTCATAG CviatR5: GTCTCCTGAATCTCTGCTTACTC CviatF3: GAGATTGTCACTCATCATCGTAC CviatR8: CTACACGTAAAATAATTCGCGAC	∼ 950	55 52	gp60	[11]
Subtyping of C. felis	GP60CF_F1: TTTCCGTTATTGTTGCAGTTGCA GP60CF_R1: ATCGGAATCCCACCATCG AAC GP60CF_F2: GGGCGTTCTGAAGGATGT AA GP60CF_R2: CGGTGGTCTCCTCAGTCTTC	∼ 900	55 55	gp60	[10]