FIG. 3.

Mapping the 1a-1a binding domain by deletion analysis. The domains of the 1a protein are depicted along with various deletions in either the N or C terminus. Fold β-galactosidase levels detected when each of the deletions was expressed with wt1a from two independent experiments are shown relative to those of wt1a alone. For strain Y187 (Clonetech Laboratories, Inc.), only the results of the qualitative filter assays are shown. Δ516-966 retains the ability to interact with both itself and wt1a. B, blue colonies; W, normal yeast colony color. AA, amino acid.