TABLE 3.
Summary of 1a intramolecular interaction occurring between methyltransferase- and helicase-like domains in BMV and CMV
| Expressed fusion proteina | Appearance of colonies in filter assayb | Growth on plates lacking histidine | Relative β-galactosidase sp actc
|
|
|---|---|---|---|---|
| Expt 1 | Expt 2 | |||
| LexA-BMT only | W | No | 1.0 | 1.0 |
| LexA-BHel only | W | No | 1.3 | 0.8 |
| LexA-BHel and GAL4-BHel | W | No | ND | ND |
| LexA-BMT and GAL4-BMT | B | Yes | 2.7 | 3.1 |
| LexA-BHel and GAL4-BMT | B | Yes | 29.6 | 24.8 |
| LexA-BMT and GAL4-BHel | B | Yes | 3.6 | 3.6 |
| LexA-wt1a and GAL4-Δ1-45 | W | No | ND | ND |
| LexA-BMT and GAL4-Δ1-45 | B | Yes | ND | ND |
| LexA-CMT only | W | No | ND | ND |
| LexA-CMT and GAL4-CMT | B | Yes | ND | ND |
| LexA-CHel and GAL4-CMT | B | Yes | ND | ND |
a
Descriptions of fusion proteins are in Table 1.
b
W, normal yeast colony color; B, blue.
c
Specific activity is shown as micromoles of O-nitrophenyl galactoside hydrolyzed per minute per milligram of protein. Data are shown as fold activity relative to the background activity of LexA-BMT alone. Results of each experiment are the average of two independent assays. ND, not determined.