Table 1.
Rats necropsied at 6 weeks postinfection; identification number (ID), sex, treatment, the observable number of adult worms found in the heart/lung complex, larvae present in lungs and feces, and real-time PCR results of brain tissue and fecal samples
| Necropsy Observations | Real-Time PCR Results | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Brain Tissue | Feces | ||||||||||||
| Rat ID | Sex | Treatment | No. Adult Worms in Heart/Lungs | Eggs or L1s Present in Heart/Lungs | L1s Present in Feces | Mean CT | CT SD | No. Pos. Reps | Result | Mean CT | CT SD | No. Pos. Reps | Result |
| Rat_025 | F | Water | 0 | No | No | 0.00 | – | 0 | N | 0.00 | – | 0 | N |
| Rat_026 | M | Water | 0 | No | No | 0.00 | – | 0 | N | 0.00 | – | 0 | N |
| Rat_027 | F | Water | 0 | No | No | 0.00 | – | 0 | N | 0.00 | – | 0 | N |
| Rat_028 | M | Water | 0 | No | No | 0.00 | – | 0 | N | 0.00 | – | 0 | N |
| Rat_098 | M | Water | 0 | No | No | 0.00 | – | 0 | N | 0.00 | – | 0 | N |
| Rat_101 | M | Water | 0 | No | No | 0.00 | – | 0 | N | 0.00 | – | 0 | N |
| Rat_108 | F | Water | 0 | No | No | 0.00 | – | 0 | N | 0.00 | – | 0 | N |
| Rat_017 | F | 0 Gy | 7 | Yes | No | 33.22 | 0.96 | 3 | P | 18.77 | 0.06 | 3 | P |
| Rat_018 | M | 0 Gy | 10 | Yes | No | 29.30 | 0.20 | 3 | P | 19.36 | 0.07 | 3 | P |
| Rat_019 | F | 0 Gy | 13 | Yes | Yes | 26.06 | 0.10 | 3 | P | 16.13 | 0.12 | 3 | P |
| Rat_020 | M | 0 Gy | 12 | Yes | No | 18.49 | 0.04 | 3 | P | 20.87 | 0.04 | 3 | P |
| Rat_046 | F | 0 Gy | 9 | Yes | No | 33.38 | 0.49 | 3 | P | 30.17 | 0.11 | 3 | P |
| Rat_051 | M | 0 Gy | 16 | Yes | Yes | 26.57 | 0.07 | 3 | P | 19.85 | 0.02 | 3 | P |
| Rat_053 | M | 0 Gy | 9 | Yes | Yes | 18.96 | 0.13 | 3 | P | 25.82 | 0.05 | 3 | P |
| Rat_001 | F | 150 Gy | 1 | No | No | 0.00 | – | 0 | N | 0.00 | – | 0 | N |
| Rat_002 | M | 150 Gy | 8 | No | No | 29.70 | 0.46 | 3 | P | 0.00 | – | 0 | N |
| Rat_003 | F | 150 Gy | 2 | No | No | 17.53 | 0.04 | 3 | P | 0.00 | – | 0 | N |
| Rat_004 | M | 150 Gy | 10 | No | No | 17.81 | 0.05 | 3 | P | 0.00 | – | 0 | N |
| Rat_036 | M | 150 Gy | 5 | No | No | 37.08 | 1.81 | 2 | P | 0.00 | – | 0 | N |
| Rat_037 | M | 150 Gy | 4 | No | No | 18.27 | 0.02 | 3 | P | 0.00 | – | 0 | N |
| Rat_038 | F | 150 Gy | 4 | No | No | 0.00 | – | 0 | N | 0.00 | – | 0 | N |
| Rat_009 | F | 400 Gy | 1 | No | No | 39.54 | 1.72 | 2 | P | 0.00 | – | 0 | N |
| Rat_010 | M | 400 Gy | 2 | No | No | 37.90 | – | 1 | E | 0.00 | – | 0 | N |
| Rat_011 | F | 400 Gy | 1 | No | No | 38.41 | 2.01 | 3 | P | 0.00 | – | 0 | N |
| Rat_012 | M | 400 Gy | 0 | No | No | 21.44 | 0.07 | 3 | P | 0.00 | – | 0 | N |
| Rat_041 | M | 400 Gy | 0 | No | No | 32.46 | 0.07 | 3 | P | 0.00 | – | 0 | N |
| Rat_045 | F | 400 Gy | 1 | No | No | 31.28 | 0.03 | 3 | P | 0.00 | – | 0 | N |
| Rat_047 | M | 400 Gy | 0 | No | No | 39.26 | 3.78 | 2 | P | 0.00 | – | 0 | N |
PCR = polymerase chain reaction. Mean cycle threshold (CT) and cycle threshold standard deviation (CT SD) are reported. All real-time PCR samples were tested in triplicate, with results of negative (N), positive (P), or equivocal (E).