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. 2024 Apr 4;4(4):986–1003. doi: 10.1158/2767-9764.CRC-23-0488

FIGURE 2.

FIGURE 2

Validation of putative protein–protein interactions identified by proximity labeling. Immunoprecipitation of DHX9 (A–C), DDX54 (D–E), or DDX17 (F–G) followed by immunoblot for ADAR in breast cancer cell lines. Immunoblot of immunoprecipitation eluates and inputs from SK-BR-3 (A, D, and F), MCF-7 (B, E, and G), and MDA-MB-231 (C). Input represents 5% of the lysate used for immunoprecipitation. The IgG lanes represent immunoprecipitation eluates from pulldown with anti-rabbit IgG antibody. The lanes labeled DHX9, DDX54, and DDX17 indicate the eluates from immunoprecipitation with antibodies against those proteins, respectively. The IgGHC label indicates the band corresponding to the IgG heavy chain from the antibody used for immunoprecipitation. Uncropped immunoblots for A–G can be found in Source Data Figures. Immunofluorescence for ADAR1 and DHX9 (H), DDX54 (I), or DDX17 (J) in SKBR3 or MCF-7.