FIG. 4.

Precipitation of ³⁵S-labeled NS1 protein by GST–NS1-BP fusion protein. Radiolabeled NS1 protein was synthesized in coupled transcription-translation reactions in the presence of [³⁵S]methionine by using pcDNA3-NS1 as a template. The NS1 protein was precipitated by glutathione-Sepharose beads coated with GST (lane GST) or GST–NS1-BP, which carries amino acids 347 to 619 of NS1-BP (lane GST–NS1-BP). The precipitates were analyzed by SDS-polyacrylamide gel electrophoresis and autoradiography. A 10% aliquot of the total reaction was separated in parallel (lane T). The positions of molecular weight markers (in thousands) are indicated to the left.