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. 2022 Feb 16;95(1133):20211150. doi: 10.1259/bjr.20211150

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© 2022 The Authors. Published by the British Institute of Radiology

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 Unported License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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Figure 3. — A) Alkaline comet assay measures of PBL DNA damage formation (% Tail DNA) following FLASH (2 kGy/s) or conventional dose rate (CONV, 0.1 Gy/s) irradiation to various total doses. Data expressed as the mean % Tail DNA of three slides (n = 3); error bars indicate standard deviation of the means for each experimental condition. Statistical analysis (two-tailed unpaired t-test) FLASH vs CONV revealed no significant differences for doses ≤ 10 Gy. However, statistically significant differences were found for 20, 30 and 40 Gy (**, p < 0.01; *, p < 0.05). Comet images of B) PBL unirradiated 0.5% O₂; C) PBL 40 Gy CONV irradiated at 0.5% O₂; D) PBL 40 Gy FLASH irradiated at 0.5% O₂. PBL, peripheral blood lymphocyte.