FIG. 1.

RV modulates NCAM expression on the cell surface. Modulation by RV of the amount of NCAM on the cell surface of BSR (A and B) and N2a (C and D) cells was analyzed by cytofluorimetry. BSR cells were either mock treated (incubation with medium alone) (solid bold line) or treated with 10 (dashed line) or 30 (solid line) PFU of RV per cell (A) or with 10 PFU of vaccinia virus per cell (dotted line) as control virus (B). The samples were then incubated with anti-NCAM MAb or culture medium (peak on the extreme left) followed by FITC-conjugated anti-mouse IgG. The specificity of NCAM modulation by RV was tested by using N2a cells, which express both NCAM and α6 chain of integrin (C and D). N2a cells were either mock treated (solid line) or treated (dotted line) with 10 PFU of RV per cell and then anti-NCAM MAb (C) or an anti-α6 chain of integrin MAb (D) or culture medium (left peak in diagrams) followed by FITC-conjugated anti-mouse IgG Ab. Values are representative of five independent experiments.