FIG. 5.

RV susceptibility of transfected NCAM cells. (A and B) Two-day cultures of RV-infected N2a, NCAM-transfected (D9 and A14), and NCAM-negative (control) cells were double stained for NCAM (red) and viral NC (green) and examined by microscopy (A) or analyzed by flow cytometry (B). RV-infected NCAM-positive cells appear as yellow-stained cells in the leftmost A panels and are located in the upper right quadrant in the B panels. (C) RV susceptibility of D9 (dashed curve), A14 (solid black curve), and control (dotted curve) cells was assessed by infection with RV (MOI of 1.5 to 0.25). Results are expressed as the percentages of cells infected 48 h after infection. (D) Inhibition of RV infection of control, D9, A14, and N2a cells by heparan sulfate and chondroitin sulfate A and B (left, middle, and right bars in each set, respectively).