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. Author manuscript; available in PMC: 2024 Apr 4.
Published in final edited form as: Vet Microbiol. 2017 Mar 4;203:68–72. doi: 10.1016/j.vetmic.2017.03.006

Fig. 3.

Fig. 3.

Blocking assay experiment. A canine serum positive for GII.4 and GVI.2 antigens with titres ≥ 1:800 was pre-incubated with GII.4 (a) and GVI.2 (b) VLPs at concentrations of 2, 4, 8 and 16 μg/ml and tested in ELISA for the presence of antibodies against both VLPs. (c) WB analysis of GII.4 and GVI.2 VP1 (at concentration of 8 mg/ml) using a human serum sample positive in ELISA for GII.4 NoV at final dilution of 1:800. (d) WB analysis of GII.4 and GVI.2 VP1 (at concentration of 8 μg/ml) using a dog serum sample positive in ELISA for GVI.2 NoV at final dilution of 1:800. Line 1: Precision Plus protein Standards (Bio-Rad, Italy); line 2: GII.4 VP1; line 3: GVI.2 VP1. Both the sera were tested in WB at dilution of 1:100.