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. 2023 Apr 28;18(4):850–865. doi: 10.1002/1878-0261.13440

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© 2023 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 6 — uLMS cell migration is not affected by collagen substrate stiffness. (A, B) Cell migration speed of leiomyoma (LM) cells (A) and uterine leiomyosarcoma (uLMS) cells (B) at indicated polyacrylamide (PAA) hydrogel stiffness (n > 10 cells/stiffness per donor; LM n = 3 donors and uLMS n = 4). (C, D) Directionality ratio of LM (C) and uLMS (D) cells at distinct PAA hydrogel stiffness (n > 10 cells/stiffness per donor; LM n = 3 donors and uLMS n = 4). (E, F) Individual cell trajectories of LM (E) and uLMS (F) cells at indicated stiffness showing relatively shorter trajectories of LM cells at 20 kPa (n > 10 cells/stiffness per donor; LM n = 3 donors and uLMS n = 4). Bar graphs represent average ± SD. All statistical analyses were performed by one‐way ANOVA with post hoc Tukey's test. *P < 0.05, **P < 0.01, ***P < 0.001.