Fig. 3.

Effects of treatment with ANP coupled HA‐GNPs on tumor formation of etoposide resistant RB cells in in ovo chorioallantoic membrane (CAM) assays. Photographs of CAM tumors in situ and ruler measurements (in cm) of excised tumors revealing that tumors forming on the upper CAM 7 days after grafting of treated Y79‐Etop (A) and WERI‐Etop (B) cells were smaller compared to those arising from control cells treated with PBS (ctr). (A) Histological analysis of paraffin sections of Y79‐Etop CAM tumors by hematoxylin and eosin (HE) and Ki67 stains (brown signal). Black arrowheads exemplarily demarcate Ki67 positive cells positive cells. Scale bars: 600 μm. (C) Quantification of the total vessel area, vessel length, thickness and branching points of HA‐GNP and ANP‐HA‐GNP treated CAM tumors compared to the controls as calculated by an ikosa online software (KLM vision). The experiments were performed in triplicates. ANP, atrial natriuretic peptide; ANP‐HA‐GNP, ANP coupled HA‐GNPs; GNP, gold nanoparticles; HA‐GNP, hyaluronic acid coupled GNPs. Values represent means of independent animals ± SEM. *P < 0.05; **P < 0.01 statistical differences compared to the control group calculated by one‐way ANOVA with Newman–Keuls post‐test.