TABLE 1.
Time after inoculation | Virus | Total no. of neurons counted | No. (%) of antigen-positive neurons | No. (%) of antigen-positive round cellsb |
---|---|---|---|---|
18 h | GDVII | 154 | 136 (88) | |
DA | 0 (0) | |||
24 h | DA | 386 | 6 (1.5) | 18 (4.7) |
48 h | DA | 460 | 10 (2.1) | 32 (6.9) |
72 h | DA | 561 | 6 (1) | 40 (7) |
6 days | DA | 568 | 13 (2.2) | 83 (14.6) |
An average of 20 fields per slide were chosen at random. Total neurons and antigen-positive neurons were counted. At 18 h postinoculation, many neurons have already been lysed by GDVII, which explains why fewer neurons could be counted.
As described in the text, antigen-positive round cells, with no morphological characteristics of neurons, were observed in cultures infected with DA virus.