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. 1998 Sep;72(9):7213–7220. doi: 10.1128/jvi.72.9.7213-7220.1998

TABLE 1.

Quantification of the number of infected neurons detected by immunofluorescence labeling of viral antigensa

Time after inoculation Virus Total no. of neurons counted No. (%) of antigen-positive neurons No. (%) of antigen-positive round cellsb
18 h GDVII 154 136 (88)
DA 0 (0)
24 h DA 386 6 (1.5) 18 (4.7)
48 h DA 460 10 (2.1) 32 (6.9)
72 h DA 561 6 (1) 40 (7)
 6 days DA 568 13 (2.2) 83 (14.6)
a

An average of 20 fields per slide were chosen at random. Total neurons and antigen-positive neurons were counted. At 18 h postinoculation, many neurons have already been lysed by GDVII, which explains why fewer neurons could be counted. 

b

As described in the text, antigen-positive round cells, with no morphological characteristics of neurons, were observed in cultures infected with DA virus.