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. 2024 Apr 4;19(4):e0301629. doi: 10.1371/journal.pone.0301629

Fig 2. The electrophoretic result of PCR-RFLP band patterns migrated on agarose gel 3.5%.

Fig 2

(A) PCR amplified BMPR1B gene digested by AvaII (5’-G/GWCC) restriction enzyme for Booroola/FecB mutation (OAR6: 29382188A>G, NC_019463.1 of genome assembly Oar_v3.1). The lanes depict Booroola mutation genotypes including homozygous reference (AA), heterozygous (AG) and homozygous variant (GG). SM: GeneRuler 1 kb Plus DNA Ladder #SM1331. (B) PCR amplified ovine myostatin gene (MSTN) digested by HpyCH4IV (5’- A/CGT) restriction enzyme for MSTN mutation (OAR2: 118150665G>A, NC_019463.1 of genome assembly Oar_v3.1). The lanes depict MSTN mutation genotypes including homozygous reference (GG), heterozygous (GA) and homozygous variant (AA). SM: GeneRuler 1 kb Plus DNA Ladder #SM1331.