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. 2024 Mar 25;18(3):e0012050. doi: 10.1371/journal.pntd.0012050

Fig 4. Identification of PHT-39 efficacy determinants in T. brucei.

Fig 4

(A) Distribution of primary read-density signatures (>50 reads) across the T. brucei genome. Selected reads are labelled (MT = miltefosine transporter Tb927.11.3350; NcA nicotinamidase) (B) Reads mapping to the MT (Tb927.11.3350) locus and (C) ESAG5 (Tb927.4.810) locus. Red and blue peaks are RNAi construct forward and reverse barcodes, respectively. Gray peaks are all other reads. (D) EC50 analyses for PHT-39 (top) and amphotericin B (bottom) after individual knockdown of MT (Tb927.11.3350). EC50 analyses were carried out in 3 biological replicates. P-values were derived from unpaired Student’s t-test (*, P < 0.01; **, P < 0.001; ***, P < 0.0001). (E) EC50 analyses for PHT-39 (top) after individual knockdown of ESAG5. EC50 analyses were carried out in 3 biological replicates. P-values were derived from a non-parametric Mann Whitney test (*, P < 0.015; **, P < 0.0079).