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. 2024 Mar 25;20(3):e1012100. doi: 10.1371/journal.ppat.1012100

Fig 4. SARS-CoV-2 DUB mutant viruses elicit a stronger immune response in Calu-3 cells.

Fig 4

Calu-3 cells were infected at MOI 0.1 with wild-type or DUB mutant SARS-CoV-2 to analyze the expression of genes related to the immune response by RT-qPCR. (A) Gene expression over time in Calu-3 cells infected with wild-type SARS-CoV-2 as compared to mock-infected cells. (B-D) Expression of genes related to the immune response at 12 (B), 24 (C), and 48 (D) hours post infection with wild-type or DUB mutant SARS-CoV-2. FEH(M) = F69S/E70K/H73G(/M208S). Panel A shows representative examples. Data in panels B-D are represented as mean ± s.e.m. of 5–8 independent experiments. Data was analyzed by mixed-effects analysis with Dunnett’s multiple comparisons test, comparing each group to wild-type SARS-CoV-2. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.