Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Mouse monoclonal anti-GFP | Molecular Probes | Cat # A11120; RPID: AB_221568 | IHC (1:1000) |
| Antibody | Rabbit polyclonal anti-DsRed | Clontech | Cat # 632496; RPID: AB_10013483 | IHC (1:1000) |
| Antibody | Goat polyclonal anti-mouse Alexa Fluro 488 |
Invitrogen | Cat # A32723; RRID: AB_2633275 | IHC (1:200) |
| Antibody | Goat polyclonal anti-rabbit Alexa Fluor 568 |
Invitrogen | Cat # A11011; RPID: AB_143157 | IHC (1:200) |
| Chemical compound | Sucrose | Sigma-Aldrich | Cat # 9378S | |
| Chemical compound | Tricholine citrate | Sigma-Aldrich | Cat # T0252 | |
| Chemical compound | Sulforhodamine B | Sigma-Aldrich | Cat # 230162 | |
| Chemical compound | Capsaicin | Sigma-Aldrich | Cat # M2028 | |
| Chemical compound | Caffeine | Sigma-Aldrich | Cat # C02750 | |
| Chemical compound | CaCl2 dihydrate | Sigma-Aldrich | Cat # C3881 | |
| Chemical compound | KCl | Sigma-Aldrich | Cat # P9541 | |
| Chemical compound | Quinine | Sigma-Aldrich | Cat # Q1125 | |
| Chemical compound | Strychnine | Sigma-Aldrich | Cat # S8753 | |
| Chemical compound | Lobeline | Sigma-Aldrich | Cat # 141879 | |
| Chemical compound | Denatonium | Sigma-Aldrich | Cat # D5765 | |
| Chemical compound | Coumarin | Sigma-Aldrich | Cat # C4261 | |
| Chemical compound | Brilliant blue FCF | Wako Pure Chemical Industry |
Cat # 027-12842 | |
| Chemical compound | Paraformaldehyde | Electron Microscopy Sciences |
Cat # 15710 | |
| Chemical compound | NaCl | LPS Solution | Cat # NACL01 | |
| Chemical compound | MgCl2 hexahydrate | SAMCHUN | Cat # M0038 | |
| Chemical compound | NaBr | DUKSAN | Cat # S2531 | |
| Chemical compound | Goat serum, New Zealand origin |
Gibco | Cat # 16210064 | |
| Genetic reagent (Drosophila melanogaster) | w1118 | Bloomington Drosophila Stock Center (BDSC) | BDSC:5905 | |
| Genetic reagent (Drosophila melanogaster) |
Ir7a1 | Dr. Y Lee Rimal et al., 2019 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir7g1: y1w*Mi{y+mDint2=MIC}Ir7gMI06687 | BDSC | BDSC:42420 | |
| Genetic reagent (Drosophila melanogaster) |
Ir7cGAL4 | Dr. MD Gordon McDowell et al., 2022 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir8a1: w*TI{w[+m*]=TI}Ir8a1;Bl1L2/CyO | BDSC | BDSC:23842 | |
| Genetic reagent (Drosophila melanogaster) |
Ir10a1: w1118Mi{GFPE.3xP3=ET1}Ir10aMB03273 | BDSC | BDSC:41744 | |
| Genetic reagent (Drosophila melanogaster) |
Ir21a1: w1118;PBac{w+mC=PB}Ir21ac02720 | BDSC | BDSC:10975 | |
| Genetic reagent (Drosophila melanogaster) |
Ir25a2 | Dr. L Vosshall Benton et al., 2009 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir47a1 | Dr. Y Lee Rimal et al., 2019 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir48a1: w1118;Mi{GFPE.3xP3= ET1}Ir48aMB09217 |
BDSC | BDSC:26453 | |
| Genetic reagent (Drosophila melanogaster) |
Ir48b1: w1118;Mi{GFPE.3xP3= ET1}Ir48bMB02315 |
BDSC | BDSC:23473 | |
| Genetic reagent (Drosophila melanogaster) |
Ir51b1: w1118;PBac{w+mC=PB}rowc00387 Ir51bc00387 | BDSC | BDSC:10046 | |
| Genetic reagent (Drosophila melanogaster) |
Ir52a1 | Dr. Y Lee Rimal et al., 2019 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir52b1: w1118;Mi{GFPE.3xP3= ET1}Ir52bMB02231/SM6a |
BDSC | BDSC:25212 | |
| Genetic reagent (Drosophila melanogaster) |
Ir52c1: w1118;Mi{GFPE.3xP3= ET1}Ir52cMB04402 |
BDSC | BDSC:24580 | |
| Genetic reagent (Drosophila melanogaster) |
Ir56a1 | Dr. Y Lee Rimal et al., 2019 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir56b1: w1118;Mi{GFPE.3xP3=ET1}Ir56bMB09950 | BDSC | BDSC:27818 | |
| Genetic reagent (Drosophila melanogaster) |
Ir56d1: w*;Ir56d1 | BDSC | BDSC:81249 | |
| Genetic reagent (Drosophila melanogaster) |
Ir60b1 | Dr. J Carlson Joseph et al., 2017 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir60b3 | Dr. Y Lee | In this study | |
| Genetic reagent (Drosophila melanogaster) |
Ir62a1: y1w*;Mi{y+mDint2=MIC} Ir62aMI00895Iml1MI00895/TM3, Sb1 Ser1 |
BDSC | BDSC:32713 | |
| Genetic reagent (Drosophila melanogaster) |
Ir67a1: y1w*;Mi{y+mDint2=MIC}Ir67aMI11288 | BDSC | BDSC:56583 | |
| Genetic reagent (Drosophila melanogaster) |
Ir75d1: w1118;Mi{GFPE.3xP3=ET1}Ir75dMB04616 | BDSC | BDSC:24205 | |
| Genetic reagent (Drosophila melanogaster) |
Ir76b1 | Dr. C Montell Zhang et al., 2013 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir85a1: w1118;Mi{GFPE.3xP3=ET1} Ir85aMB04613 Pif1AMB04613 |
BDSC | BDSC:24590 | |
| Genetic reagent (Drosophila melanogaster) |
Ir92a1: w1118;Mi{GFPE.3xP3=ET1}Ir92aMB03705 | BDSC | BDSC:23638 | |
| Genetic reagent (Drosophila melanogaster) |
Ir94a1 | Dr. Y Lee Rimal et al., 2019 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir94b1:w1118;Mi{GFPE.3xP3= ET1}Ir94bMB02190 |
BDSC | BDSC:23424 | |
| Genetic reagent (Drosophila melanogaster) |
Ir94c1 | Dr. Y Lee Rimal et al., 2019 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir94d1:y1w*;Mi{y+mDint2=MIC} Ir94dMI01659CG17380MI01659 |
BDSC | BDSC:33132 | |
| Genetic reagent (Drosophila melanogaster) |
Ir94f1: y1w*;Mi{y+mDint2= MIC}Ir94fMI00928 |
BDSC | BDSC:33095 | |
| Genetic reagent (Drosophila melanogaster) |
Ir94g1: w1118;Mi{GFPE.3xP3= ET1}Ir94gMB07445 |
BDSC | BDSC:25551 | |
| Genetic reagent (Drosophila melanogaster) |
Ir94h1 | Dr. Y Lee Rimal et al., 2019 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir100a1: w1118;P{w+mC=EP} Ir100aG19846 CG42233G19846 |
BDSC | BDSC:31853 | |
| Genetic reagent (Drosophila melanogaster) |
UAS-mCD8::GFP | BDSC | BDSC:5137 | |
| Genetic reagent (Drosophila melanogaster) |
UAS-mCD8::GFP | BDSC | BDSC:32184 | |
| Genetic reagent (Drosophila melanogaster) |
UAS-Kir2.1 | BDSC | BDSC:6595 | |
| Genetic reagent (Drosophila melanogaster) |
UAS-Ir25a | Dr. Y Lee Lee et al., 2018 | ||
| Genetic reagent (Drosophila melanogaster) |
UAS-Ir60b | Dr. Y Lee | In this study | |
| Genetic reagent (Drosophila melanogaster) |
UAS-Ir76b | Dr. C Montell Zhang et al., 2013 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir25a-GAL4 | Dr. L Vosshall Benton et al., 2009 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir60b-GAL4 | Dr. C Montell Joseph et al., 2017 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir76b-GAL4 | Dr. C Montell Zhang et al., 2013 | ||
| Genetic reagent (Drosophila melanogaster) |
ppk23-GAL4 | Dr. K Scott Thistle et al., 2012 | ||
| Genetic reagent (Drosophila melanogaster) |
ppk28-GAL4 | Dr. H Amrein Cameron et al., 2010 | ||
| Genetic reagent (Drosophila melanogaster) |
Gr66a-GAL4 | Dr. H Amrein Thorne et al., 2004 | ||
| Genetic reagent (Drosophila melanogaster) |
Gr64f-GAL4 | Dr. A Dahanukar Lee et al., 2018 | ||
| Genetic reagent (Drosophila melanogaster) |
Ir76b-QF | BDSC | BDSC:51312 | |
| Genetic reagent (Drosophila melanogaster) |
QUAS-tdTomato: y1w1118; P{QUAS-mtdTomato-3xHA}26 |
BDSC | BDSC:30005 | |
| Genetic reagent (Drosophila melanogaster) |
Poxn∆M22-B5: y1w67c23; Mi{ET1}PoxnMB00113 |
BDSC | BDSC:22701 | |
| Genetic reagent (Drosophila melanogaster) |
Poxn70-28: Poxn70/CyO; twi-Gal4, UAS-2XEGFP |
BDSC | BDSC:60688 | |
| Software | Origin Pro Version | Dr. Y Lee | https://www.originlab.com | |
| Software | GraphPad Prism | Dr. Y Lee | https://www.graphpd.com | |
| Software | Autospike 3.1 software | Dr. Y Lee | https://www.syntech.co.za/ | |
| Software | Fiji/ImageJ software | Dr. Y Lee | https://fiji.sc | |
| Software | ZEN lite 2.5 blue | Dr. Y Lee | https://www.zeiss.com/ |