Fig. 6. Inhibition of PG synthesis slows down the directional movement of FtsW and DivIB.
a–e, Histograms of FtsW-HT (a, c and e) and HT-DivIB (b and d) single-molecule velocities determined in JE2 EzrA–sGFP and COL EzrA–sGFP backgrounds in the absence and presence of β-lactam imipenem or MurJ inhibitor DMPI (a and b), in PBP1 TPase mutant ColPBP1TP EzrA–sGFP and COL EzrA–sGFP backgrounds (c and d), and in JE2 EzrA–sGFP background producing either FtsW-HT wild type or its active-site mutant derivatives W121A and D287A from the ectopic spa locus (e). Strains were grown in TSB rich medium at 37 °C. Average velocity is shown as mean with standard deviation. Bin width, 4. Centre of first/last bin, 2/42. f, FtsW-HT and HT-DivIB mean velocities shown in a,c and b,d, respectively, as a function of septum constriction rate shown in Fig. 1 and Supplementary Table 1. Horizontal error bars represent the standard deviations of 20 cells and vertical error bars represent the standard deviations of a minimum of 164 trajectories from three biological replicates.