TABLE 3.

EIAV RNA in tissues and plasma, detected by RT-PCR and ISH

Horse no.	Clinical status	Virus strain	RT-PCR (nested PCR)a					ISH, gag in spleen
			Spleen		Bone marrow		gag in plasma
			gag	env	gag	env
2084	Acute clinical	Wyo	+	+	ND	ND	+	+
2079	Acute clinical	WSU5	+	+	ND	ND	+	+
2085	Chronic clinical	Wyo	+	+	+	− (+)	+	+
524	Subclinical	Wyo	+	− (+)	− (+)	− (+)	− (+)	+
489	Subclinical	WSU5	− (+)	− (+)	ND	ND	−b	−
498	Subclinical	WSU5	− (+)	− (+)	ND	ND	−b	−

^a+, positive PCR result detected in ethidium bromide-stained agarose gels; −, negative result; ND, not done. 

^bUltracentrifuge pellets from 4 ml of plasma were pooled for nested RT-PCR.