FIGURE 1.
(A) The workflow used to perform our study in a best practice approach. Orange lightning bolts indicate steps found to differ from the prior investigation of the MC metagenome by Rajasekaran et al., 9 while red indicates steps done only in this workflow and not in prior MC microbiome studies. Blue lightning bolts indicate the steps which were further investigated with the use of the dataset from this study. (B) Direct comparison of the number of genera detected in our study compared to the number presented by Rajasekaran et al. 9 Different parameters were used for the analysis of our samples including the use of either ASVs or OTUs as well as the application of two different prevalence cut‐off filters for the number of samples indicating the presence of these genera. ASV, amplicon sequencing variant; OTU, operational taxonomic unit.