Fig. 1.

(A) Images of the injection molding machine equipped with a Master Unit Die (MUD) consisting of a movable and a stationary platens. A blank mold insert was placed on the surface of the moving platen while a resin mold insert was fixed to the stationary platen. (B) SEM images of the Si master used for nanoscale electrophoresis, which consist of dual in-plane nanopores flanked into both ends of a nanochannel. (C) SEM images of a nanofluidic structure used for selective binding of a single RNA molecule to a solid phase bioreactor. The structure consist of a nanopillar in the center which is connected to four input/output nanochannel of ∼250 nm × 250 nm in width × depth and one nanochannel of ∼50 nm × 50 nm in width × depth to capture the reaction products from the solid phase reactor. (D) and (E) AFM images of replicated MD700 molds with positive and negative toned structures fabricated by repetitive replication from Si master with the dual in-plane nanopore Time-of-Flight (ToF) sensor structure.