Fig. 4.

RNA translocation through an injection molded single-molecule sequencing device. (A) Experimental setup showing the electrical connections to the chip with a waveform generator for supplying the electrical field for driving the RNA (CAS9) through the chip. (B) Rapid scanning confocal image of the single-molecule sequencing device with the yellow box showing the area that is imaged with the single-molecule laser-induced fluorescence tracking microscope. (C) Fluorescence image Syto 82 labeled RNA electrically translocating through the input/output channels of the mixed-scale sequencing device. In this case, there was no ribo-exonuclease covalently attached to the solid-phase bioreactor portion of the device. Also, this device did not contain the in-plane nanopores within the input/output channel network. (D) Same conditions as shown and discussed in (C), but in this case, there was XRN1 ribo-exonuclease attached to the solid-phase bioreactor, which associates to the translocating RNA molecule causing it to remain stationary.