Figure 2.

DNAJA1-KO inhibits HS-induced apoptosis of HUVECs. HUVECs were exposed to 43°C for 4 h and prepared for testing immediately thereafter. (A) Apoptosis was analyzed by flow cytometry using Annexin V-FITC/PI staining. HS-induced apoptosis was markedly inhibited by DNAJA1-KO. (B) Expression levels of Bax, Bcl-2, caspase-3 and cleaved caspase-3 were measured by western blotting. (C) The apoptosis rate of cells in CON + HS was significantly increased compared with cells in CON, while only a few cells were apoptotic in KO + HS. Representative data are presented as the mean ± SEM (n=3). *P<0.05, **P<0.01. ns, not significant; KO, knockout; CON, control; HS, heat stroke; HUVECs, human umbilical vein endothelial cells.