Figure 3.

DNAJA1-KO alleviates HS-induced endothelial barrier dysfunction in the HUVEC monolayer. Endothelial monolayer samples were cultured at 43°C for 4 h and experiments were conducted on confluent HUVECs monolayers after a stable TEER was recorded. (A) TEER was monitored at the indicated time points and was considered to form the integrated endothelial monolayer when the values remained stable after increasing. (B) TEER in each group was detected and compared after 4 h of HS treatment. (C) Concentrations of FITC-Dextran in the bottom chamber were measured and compared after 4 h of HS treatment. (D) Expression levels of ZO-1, JAM-A, Claudin-5 and occludin were analyzed by western blotting, with GAPDH used as the loading control. Representative data are presented as the mean ± SEM (n=3). *P<0.05, **P<0.01, ***P<0.001. ns, not significant; KO, knockout; TEER; transendothelial electrical resistance; HS, heat stroke; CON, control; HUVECs, human umbilical vein endothelial cells; ZO-1, zonula occludens-1; JAM-A, junctional adhesion molecule A.