Figure 8.

DNAJA1-KO alleviates multiple-organ injury and lung endothelial barrier dysfunction in HS mice. Blood samples of mice in each group were collected 3 h after HS induction. Serum biochemical indices of (A) ALT, (B) AST, (C) BU, (D) CK and (E) CREA were examined by automatic biochemical analyzer. Serum (F) VWF, (G) TM and (H) ET-1 were detected by ELISA kits. Representative histopathological images are shown of lungs from (I) CON, (J) DNAJA1-KO, (K) CON + HS and (L) KO + HS mice (magnification, ×100; scale bar, 100 µm). (M-P) VE-cadherin was stained with the corresponding antibody (red) and cell nuclei were stained using DAPI (blue; scale bar, 100 µm) in (M) CON, (N) DNAJA1-KO, (O) CON + HS and (P) KO + HS (magnification, ×100; scale bar, 100 µm). Representative data are presented as the mean ± SEM (n=3). *P<0.05, **P<0.01, ***P<0.001. ns, not significant; KO, knock out; HS, heat stroke; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BU, blood urea; CK, creatine kinase; CREA, creatinine; VWF, von Willebrand factor; TM, thrombomodulin; ET-1, endothelin-1; CON, control; H&E, hematoxylin and eosin.