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Elution of alphavirus from heparin-Sepharose columns. Virus (70,000 to 100,000 cpm) was added to the column in 100 mM NaCl buffer and eluted with a gradient of from 100 to 500 mM NaCl. (A) SV. (B) RRV. Single viral clones from RRV peak fractions 10 and 17 were selected, grown, and radiolabeled. These viral clones eluted at salt concentrations similar to the peaks from which they were originally taken (C and D).
