FIG. 6.

Infectivity of TR339, TRSB, and TRSB-R114 GFP-expressing RP and binding of analogous viruses to wild-type CHO-K1 cells and CHO cell mutants deficient in all GAGs (pgsD-745) or HS (pgsD-677). (A) Monolayers of each cell type were infected (60 min) with 100 to 200 BHK GFP-infectious units followed by three washes with VB, medium replacement, and incubation for 8 to 12 h. Cells expressing GFP were enumerated by fluorescence microscopy. Data are averages of four infections of TR339 (hatched bars), TRSB (solid bars), and TRSB-R114 (cross-hatched bars). (B) Suspension binding assays (10⁵ cpm added per reaction) were completed as described for BHK cells. Results for TR339 (hatched bars), TRSB (solid bars), and TRSB-R114 (cross-hatched bars) are presented as percent cpm bound and represent averages of two or three reactions.