Table 1.
Composition of the 2.0 and 2.9 microglia differentiation media
| 2.0 microglia differentiation medium | 2.9 microglia differentiation medium | Catalog number |
|---|---|---|
| DMEM/F12 | - | 11039047 (Thermo Fisher Scientific) |
| - | MEMα | 12571063 (Thermo Fisher Scientific) |
| GlutaMAX 1X | GlutaMAX 1X | 35050061 (Thermo Fisher Scientific) |
| Non-essential amino acids 1X | - | 11140050 (Thermo Fisher Scientific) |
| B27 2X | B27 2X | 17504044 (Thermo Fisher Scientific) |
| N2 0.5X | - | 17502048 (Thermo Fisher Scientific) |
| Insulin-Transferrin-Selenium 2X | Insulin-Transferrin-Selenium 2X | 41400045 (Thermo Fisher Scientific) |
| Monothioglycerol 400 μM | - | 88640 (Sigma-Aldrich) |
| Penicillin/Streptomycin 1X | Penicillin/Streptomycin 1X | 15140122 (Thermo Fisher Scientific) |
| IL-34* 100 ng/mL | IL-34* 100 ng/mL | 200–34 (Peprotech) |
| TGF-β1* 50 ng/mL | TGF-β1* 50 ng/mL | 100–21 (Peprotech) |
| M-CSF* 25 ng/mL | M-CSF* 25 ng/mL | 300–25 (Peprotech) |
| - | GM-CSF* 5 ng/mL | 300–03 (Peprotech) |
| CX3CL1*,# 100 ng/mL | CX3CL1*,# 100 ng/mL | 300–18 (Peprotech) |
| CD200*,# 100 ng/mL | 174024 (Abcam) |
*Freshly added, #from day 24 onward