Fig. 6.

Digital spatial proteomic analysis of cases with Lewy body pathology and Controls in frontal cortex. a Selected region of interests (ROIs) from tissue with Lewy body pathology (MIX, LBD) and Control. For the selection of pathology, circles of 200 μm were drawn around Lewy body bearing neurons and neurons without pathology. Morphology markers α-Syn (NanoString α-Syn Alexa Fluor® 594) (red) and α-MAP-2 (NanoString Alexa Flour® 532) (green) were used to distinguish neurons with accumulations of α-Syn in form of Lewy bodies (arrows). DNA was stained with SYTO13 dye (blue). b Volcano plot for the expressed proteins in cases with Lewy body pathology (MIX and LBD), comparison of the microenvironment of Lewy body bearing neurons and neurons without pathology. c Volcano plot for the expressed proteins in the microenvironment of non-Lewy body bearing neurons in disease (MIX and LBD) and Control cases. In volcano plots, unadjusted p‐value of 0.05 and fold‐change (FC) of 1.5 were used to identify differentially expressed proteins. Color code of detected proteins based on used NanoString Antibody Panels: blue – AD, red – Astrocyte/Inflammation, green – PD, pink – Microglia, turquoise – Disease-Associated Microglia, dark grey – Microglia/Inflammation, light grey – other