Fig. 1.

Identification of UCMSCs, and the transfection efficiency of the LV-hsa-miR-21-5p/LV-hsa-miR-21-5p-inhibition into UCMSCs. A Black histograms represent the expression of UCMSCs specific surface marker. B The cultured UCMSCs present as the fibroblast-like morphology. C and D UCMSCs differentiated into osteoblasts or lipoblasts in specific conditions. Osteoblasts are displayed by Alizarin Red staining and darker red staining indicates calcium deposition (C). Lipoblasts were displayed by the accumulation of neutral lipid vacuoles stained with Oil Red O (D). E and F The GFP staining of miR-21 in UCMSCs transfected with LV-hsa-miR-21-5p/LV-hsa-miR-21-5p-inhibition/LV-NC. G The transfection efficiency of the LV-hsa-miR-21-5p/LV-hsa-miR-21-5p-inhibition into UCMSCs. ^*P < 0.05, ^***P < 0.001 vs. LV-NC-UCMSCs group