Table 1.
Sequences of the primers used in QRT-PCR.
| Target gene | Primer | Nucleotide sequence |
|---|---|---|
| h-Rnu6(U6) | F | 5’-TGCGGGTGCTCGCTTCGGCAGC-3’ |
| R | 5’-CCAGTGCAGGGTCCGAGGTA-3’ | |
| h-miR-21 | F | 5’-TAGCTTATCAGACTGATGTTG-3’ |
| R | 5’-GCTGTCAACGATACGCTACGTAACG-3’ | |
| mus-Rnu6(U6) |
F R |
5’-CTCGCTTCGGCAGCACA-3’ 5’-AACGCTTCACGAATTTGCGT-3’ |
| mus-miR-21 |
F R |
5’-ACACTCCAGCTGGGTAGCTTATCAGACTGATG-3’ 5’-CTTAACGGCTGAGGTGCTGT-3’ |
| mus-priMir21 |
F R |
5’-GACATCGCATGGCTGTACCA-3’ 5’-CCATGATTCAACAGTCAACATCA-3’ |
| mus-GAPDH | F | 5’-AGGTCGGTGAACGGATTTG-3’ |
| R | 5’-TGTAGACCATGTAGTTGAGGTCA-3’ | |
| mus-PTEN | F | 5’-TGGATTCGACTTAGACTTGACCT-3’ |
| R | 5’-TGGCGGTGTCATAATGTCTCT-3’ | |
| mus-FOXO3a | F | 5’-CTGGGGGAACCTGTCCTATG-3’ |
| R | 5’-TCATTCTGAACGCGCATGAAG-3’ | |
| mus-caspase3 | F | 5’-TGGTGATGAAGGGGTCATTTATG − 3’ |
| R | 5’-TTCGGCTTTCCAGTCAGACTC-3’ |