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. 2024 Apr 5;12(4):e1234. doi: 10.1002/iid3.1234

Figure 5.

Figure 5

LINC01686 regulates LPS‐induced expression of IL‐6 and IL‐8 via the miR‐18a‐5p/A20/STAT1 axis. (A–C) THP‐1 cells were transfected with LINC01686 siRNA or LINC01686 fragments as indicated. After 48 h, expression levels of A20 mRNA (A) (n = 3) and STAT1 mRNA (B) (n = 3) were determined by qRT‐PCR, and the protein levels of A20, STAT1, and actin were evaluated by Western blot (C) (n = 1). (D and E) Cells transfected as in A were stimulated with LPS for 24 h, and the production levels of IL‐6 and IL‐8 in the culture supernatant were determined by ELISA (n = 3). **p < .01, ***p < .001. ELISA, enzyme‐linked immunosorbent assay; IL, interleukin; LPS, lipopolysaccharide; mRNA, messenger; qRT‐PCR, quantitative reverse transcription PCR; siRNA, small interfering RNA; STAT‐1, signal transducer and activator of transcription 1.