Table 1.

Common fixative methods used for developing vertebrate embryos.

Fixative Chemical	Chicken	Zebrafish	Frogs
Aldehyde-based	(Balint and Csillag, 2007; Carro et al., 2013; Chacon and Rogers, 2019; Rogers et al., 2013a; Rogers et al., 2013b)	(Hammond-Weinberger and ZeRuth, 2020; Macdonald, 1999; Shimomura et al., 2007)	(Acton et al., 2005; Fagotto and Brown, 2008; Kurth et al., 2010; Lee et al., 2008; Robinson and Guille, 1999; Tao et al., 2007)
Alcohol-based			(Lee et al., 2008; Ossipova et al., 2022; Ossipova and Sokol, 2021)
Acid-based		(Lasseigne et al., 2021; Macdonald, 1999; Martin et al., 2022)
Other			(Acton et al., 2005)