Table 1.
Substrate Competition Assay
| Equimolar Enzyme Mixture | Products Formeda | Product Ratio from GGPP | Product Ratio from DMAPP and IPP |
|---|---|---|---|
| PaFS, CotB2 | FS:CO | 6.7 ± 0.8 : 1 | 52.7 ± 1.6 : 1 |
| PaFS, SpS | FS:SP | 5.7 ± 0.4 : 1 | 74 ± 9 : 1 |
| PaFSLL, CotB2 | FS:CO | 3.2 ± 0.4 : 1 | 27 ± 5 : 1 |
| PaFSLL, SpS | FS:SP | 4.2 ± 0.6 : 1 | 19 ± 3 : 1 |
| CotB2-PaFSPT, PaFSCY | FS:CO | 3.3 ± 1.7 : 1 | 83 ± 5 : 1 |
| CotB2-PaFSPT, SpS | CO:SP | 1.2 ± 0.1 : 1 | 0.3 ± 0.1 : 1 |
| PaFSPT, PaFSCY, CotB2 | FS:CO | 3.0 ± 0.3 : 1 | 12.9 ± 1.8 : 1 |
| PaFSPT, PaFSCY, SpS | FS:SP | 8.1 ± 0.7 : 1 | NDb |
| PaFSPT, CotB2, SpS | CO:SP | 1.8 ± 0.7 : 1 | 1.4 ± 0.6 : 1 |
| PaFS, CotB2-PaFSPT | FS:CO | 3.4 ± 0.3 : 1 | 63.7 ± 1.7 : 1 |
| PaFS D474A, CotB2-PaFSPT | FS:CO | 2.8 ± 0.1 : 1 | 47.9 ± 1.7 : 1 |
| PvCPSPT, PaFSCY, CotB2 | FS:CO | 4.3 ± 0.3 : 1 | 42.6 ± 1.2 : 1 |
| PvCPSPT, PaFSCY, SpS | FS:SP | 6.6 ± 1.4 : 1 | 68.7 ± 4.4 : 1 |
| PvCPSPT, CotB2, SpS | CO:SP | 1.2 ± 0.2 : 1 | 0.4 ± 0.1 : 1 |
a
FS, fusicoccadiene; CO, cyclooctatenol; SP, spatadiene.
b
ND, spatadiene not detected.