Figure 6. N-terminal polybasic domains across septins.

(A) Calculation of isoelectric point windows across the NTE of reconstructed ancestral sequences. The average isoelectric point of a sliding 10 amino acid window is calculated across the NTE of reconstructed ancestral sequences. X=0 represents the start of the GTPase domain. (B) CLUSTALw multiple sequence alignment of reconstructed ancestral sequences displaying two polybasic domains in non-opisthokont lineages. Numbers indicate the amino acid positions from the start of the GTPase domain.