Figure S4.

Further characterization of AP-1 cargo constructs. (A) Western blots comparing expression levels of GFP- and HaloTag-constructs. The cells were all incubated with biotin for 40 min. In every case, the constructs labeled with GFP are expressed at ∼threefold higher levels than the same constructs labeled with HaloTag. (B) Fluorescent images of cells coexpressing GFP-CDMPR and HaloTag-CDMPR. Although there is variability in relative expression levels when different cells are compared, the fine details are virtually identical (insets). Scale bars: 10 μm. Source data are available for this figure: SourceData FS4.