Figure S5.

Protein rank plots. The graphs show the mean rank (0 is lowest intensity) versus the mean log₁₀ intensity for each of the 632 proteins analyzed in Fig. 9. The L (light) channel is the input, i.e., the total CCV-enriched fraction, while the H (heavy) channel is the bead-captured proteins. CHC17 (CLTC) is the most abundant protein in both channels and ribosomal proteins (black) are also abundant in both channels. However, AP-1, as well as AP-1-associated proteins identified in our previous knocksideways study (red) (Hirst et al., 2012), shift up the rank distribution in the heavy channel. The L (light) channel (A) is the input, i.e., the total CCV-enriched fraction, while the H (heavy) channel (B) is the bead-captured proteins.