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Cell coexpressing mRuby2-AP-1 (red), SBP-HaloTag-NAGPA (green), and SBP-GFP-CDMPR (blue), and treated with biotin for 27 min. AP-1 puncta can be seen forming on NAGPA-positive tubules. Small amounts of CDMPR are also in the tubules, but most of the CDMPR leaves in vesicular carriers. The arrowhead on the left shows an AP-1-positive NAGPA tubule being pulled out from the Golgi; the one on the right shows a fast-moving AP-1-positive NAGPA tubule. Playback speed, 28x.
