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Cell coexpressing mRuby2-AP-1 (red) and SBP-GFP-NAGPA (green), fed far-red transferrin (blue) for 1 h, and treated with biotin for 27 min. AP-1 puncta can be seen on both NAGPA-positive tubules and transferrin-positive tubules (arrowheads). In the region indicated with the upper arrowhead, the two types of tubules appear possibly to merge. Playback speed, 28x.
