FIG. 2.

Us9 protein in PRV Be- and Ba-infected cell lysates and virions. Monolayers of PK15 cells were infected with PRV Be or Ba virus at an MOI of 10. PRV-infected monolayers were radiolabeled for 11 h beginning at 5 h postinfection. (A) Cell lysates were either denatured with SDS and dithiothreitol or left untreated prior to immunoprecipitation with Us9 antiserum or preimmune serum. The identity of the polypeptide in nondenatured Be lysates immunoprecipitated with either Us9 antiserum or preimmune serum (indicated by an asterisk) is currently unknown. (B) Radiolabeled PRV virions were collected at 16 h postinfection by centrifugation through a 30% sucrose cushion and subjected to immunoprecipitation with Us9 antiserum. Positions of molecular mass markers (kilodaltons) are indicated on the left.