FIG. 5.

Immunofluorescence analysis of Us9 in PRV Be-infected cells. PK15 cells grown on glass coverslips to approximately 70% confluency were infected with PRV Be at an MOI of 10. At 2.5, 3, 4, and 6 h postinfection (hpi), the cells were fixed with formaldehyde and processed to detect Us9 protein by indirect immunofluorescence and confocal microscopy. A fluorescein isothiocyanate-conjugated donkey anti-rabbit immunoglobin G secondary antibody was used to visualize the Us9 antigen-antibody complexes.